ISOLATION OF HEXOKINASE FROM BAKER'S YEAST
نویسندگان
چکیده
منابع مشابه
Isolation of Hexokinase from Baker's Yeast
1. A method is described for the isolation of hexokinase from baker's yeast. The method is based mainly on fractionation with alcohol and results See PDF for Structure in a 30-fold increase in specific activity. The final product could be crystallized from ammonium sulfate without change in specific activity. 2. The enzyme catalyzes a transfer of phosphate from adenosinetriphosphate to glucose,...
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has been obtained in pure form from baker 's yeast by a method based chiefly on fractional precipitation with alcohol. 1 mg. of the pure protein catalyzes the transfer of 4.2 mg. P per minute at 30 ° and pH 7.5. The reported crystallization of the protein from dilute alcohol is not readily reproducible. Crystallization from ammonium sulfate (Fig. 1) according to the procedure of Kunitz and McDo...
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Hexokinase has been isolated from baker's yeast and crystallized (Fig. 1). Three other proteins of yeast were also crystallized during the course of this work (Figs. 2 to 4). One of these proteins is yellow (Fig. 4) and has a prosthetic group closely related to flavin. No enzymatic activity could be detected in the case of these three proteins.
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1946
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.29.6.379